Protein_Domain

Part:BBa_K4316019

Designed by: Luca Donati   Group: iGEM22_Sorbonne_U_Paris   (2022-10-06)


UVR8 CDS B3

UVR8 CDS was designed to be used with the Chlamydomonas Reinhardtii MoClo kit [1] and is in position B3. Its 5’ scar is A (because start codon ATG is contained in this sequence) and its 3’ scar is AGGT.

The UVR8 protein serves as a regulator of the transcription of a set of genes [2], and dimerizes with COP1 under UV radiation

Thymidine in position 18 was replaced with cytidine as to remove PstI site and make part compatible with RFC10 standard.

Sources:


[1] Crozet, Pierre & Navarro, Francisco & Willmund, Felix & Mehrshahi, Payam & Bakowski, Kamil & Lauersen, Kyle & Pérez-Pérez, María & Auroy, Pascaline & Gorchs Rovira, Aleix & Sauret-Gueto, Susana & Niemeyer, Justus & Spaniol, Benjamin & Theis, Jasmine & Trösch, Raphael & Westrich, Lisa & Vavitsas, Konstantinos & Baier, Thomas & Hübner, Wolfgang & de Carpentier, Félix & Lemaire, Stéphane. (2018). Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology. 7. 10.1021/acssynbio.8b00251.

[2] Jenkins GI. The UV-B Photoreceptor UVR8: From Structure to Physiology. Plant Cell. 2014;26: 21–37. doi:10.1105/tpc.113.119446


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 839
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
    Illegal AgeI site found at 661
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None